|Individual stars collectible||106|
|Overall stars collectible||101|
The Pacifist Challenge is a challenge to get as many stars as possible without killing any enemies. Note that we do not include Bowser because he is required to beat to get the Grand Star, giving it no potential to beat without killing an enemy.
These are stars that are impossible regardless of a full-game run or in isolation.
- Big Bob-omb on the Summit - Requires killing King Bob-omb
- Chip Off Whomp's Block - Requires killing King Whomp
- All of Big Boo's Haunt requires killing a Boo in the courtyard. Even if you were to exclude this, the following 5 stars are impossible anyway:
- Go on a Ghost Hunt - Requires killing five Boos and one Big Boo
- Ride Big Boo's Merry Go Round - Requires killing five Boos and one Big Boo. Additionally, Go on a Ghost Hunt must be completed to select this star.
- Big Boo's Balcony - Requires killing one Big Boo
- Eye to Eye in the Secret Room - Requires killing one Mr. I
- Big Boo's Haunt 100 Coins - Only 57 non-enemy coins (72 if Bookends don't count as enemies) with no infinite coin glitches.
- Hazy Maze Cave 100 Coins - Only 92 non-enemy coins with no infinite coin glitches.
- Boil the Big Bully - Requires killing one Big Bully.
- Bully the Bullies - Requires killing 3 Bullies and one Big Bully.
- In the Talons of the Big Bird - Requires killing Klepto
- Stand Tall on the Four Pillars - Requires killing Eyerok.
- Chill with the Bully - Requires killing a Chill Bully.
- Pluck the Piranha Flower - Requires killing 5 Piranha Plants.
- Make Wiggler Squirm - Requires killing Wiggler
- Tiny-Huge Island 100 Coins - Not enough non-enemy coins and only infinite coin glitch requires killing Piranha Plants.
These are stars that are possible in isolation, but are impossible in a full-game run.
- Footrace with Koopa the Quick - Requires selecting star 2
- To the Top of the Fortress - Requires selecting star 2
- Secret of the Haunted Books - Requires killing a Boo to enter
- Seek the 8 Red Coins - Requires killing a Boo to enter
- Rematch with Koopa the Quick - Requires selecting star 3